BioWrite™ micropatterned substrates
Examples of cells cultured on Intelligent Substrates' standard and custom protein micropatterns. Click here for a Flash slideshow
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Swiss 3T3 fibroblasts on fibronectin grids, line widths are 8 microns.

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Swiss-3T3 fibroblast on 10 µm squares of fibronectin.

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HUVECs on 50 µm wide lines of fibronectin.

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MCF7 cell stradling 40 µm squares of fibronectin (green). Actin is red, and nucleus is blue.

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Swiss 3T3 fibroblasts on fibronectin triangles.

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Swiss 3T3 fibroblasts on fibronectin triangles.

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Swiss 3T3 fibroblasts on fibronectin diamonds.

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Swiss-3T3 fibroblasts on 60 µm circles of fibronectin.

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HUVECs on 50 µm grid of fibronectin (green). Actin is red. Nuclei are blue.

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Swiss 3T3 fibroblasts on 50 µm collagen squares.

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Swiss 3T3 fibroblasts on fibronectin-patterned PDMS.

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Complex fibronectin pattern.

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Swiss-3T3 fibroblasts on 20 µm lines of collagen Type 1.

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Swiss-3T3 cells on an array of 5 µm squares of fibronectin. Actin is blue, and vinculin is green. Note focal adhesions that are localized to the FN squares appear yellow.

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MDA-MB-231 cells on 30µm lines of FN. Cell membrane is red.

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WI-38 human lung fibroblasts on 50 µm squares of fibronectin.

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Swiss-3T3 cells confined to 50 µm FN square patterns. Actin is red.

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HUVECs on 50 µm grid of fibronectin (green). Actin is red. Nuclei are blue.

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Swiss-3T3 cells confined to a grid of 50 µm squares. Cell membrane is red. Unconfined cells vary in size, shape, and location.

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WI-38 human lung fibroblasts on 70 µm lines of fibronectin (greeen). Actin is red, nuclei are blue.

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Swiss-3T3 cells confined to a grid of 50 µm squares. Cell membrane is red.

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CHO cells on 35 µm lines of fibronectin.

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Swiss-3T3 cells align to 30 µm FN line patterns. Actin is red.

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MDA-MB-231 breast cancer cells on 10 µm lines of fibronectin.

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HUVECs on 50 µm grid of fibronectin (green). Actin is red. Nuclei are blue.

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Swiss 3T3 fibroblasts on 70 µm lines of fibronectin (greeen). Actin is red, nuclei are blue.

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PC12 cells on 50 µm ECM squares.

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WI-38 cells on narrow lines of fibronectin.

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WI-38 uhman lung fibroblasts on 20 µm lines of fibronectin.

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Swiss-3T3 fibrblasts on variety of fibronectin patterns. The squares in the lower right are 50 µm on a side. Actin is red.

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Swiss-3T3 fibroblasts on fibronectin circle with 50 µm diameter.

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Phase image of Swiss-3T3 cells on 50 µm squares of fibronectin.

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PC12 cells on 50 µm squares of ECM proteins. Collagen is green, actin is red and nuclei are blue.

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Human umbilical vein endothelial cells (HUVECs) on 50 µm grid of fibronectin (green). Actin is red. Nuclei are blue.